Receptor-Targeted Gene Delivery Using Multivalent Phagemid Particles
نویسندگان
چکیده
منابع مشابه
Cell-targeted phagemid particles preparation using Escherichia coli bearing ligand-pIII encoding helper phage genome.
Benchmarks The filamentous phages M13, f1, and fd have been widely applied in biological research such as phage display library, phage gene delivery, and antigen preparation (1). For phagemid-mediated gene delivery, we have previously reported that modified helper phage M13KO7P1, which encodes a 12-mer ligand peptide and pIII fusion, could be used to rescue phagemid that encodes the gene of int...
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Ligand or peptide-targeted phagemid particles are being pursued as vehicles for receptor-mediated gene delivery. Here we describe a helper phage in which the protein III (pIII) protein is modified by the addition of a ligand peptide sequence at the amino terminus. Phagemid particles can be prepared with the help of this modified helper phage and should display the ligand peptide in most of the ...
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ژورنال
عنوان ژورنال: Molecular Therapy
سال: 2001
ISSN: 1525-0016
DOI: 10.1006/mthe.2001.0284